Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
Olgu Enis Tok1,2, Gamze Demirel2,3, Yusuf Saatci1, Zeynep Akbulut3,5, Ozgecan Kayalar6, Ranan Gulhan Aktas2,3,4
1Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 2Cellorama, 3Cancer and Stem Cell Research Center, Maltepe University, 4Department of Histology and Embryology, School of Medicine, Maltepe University, 5Department of Medical Biology, School of Medicine, Maltepe University, 6School of Medicine, Koc University
The present study describes methodologies for culturing, freezing, thawing, processing, staining, labeling, and examining entire spheroids and organoids under various microscopes, while they remain intact in a hydrogel within a multipurpose device.